Magnetic spermatic separation with annexin columns

We distinguish healthy and quality sperm for best results

Tecnology

Magnetic spermatic separation with annexin columns

What is it?

The presence of a high quantity of spermatozoa with altered plasma membrane and fragmented DNA, has a negative impact on natural fertility and also on the results of assisted reproduction. For this reason, having an effective molecular filtering method, capable of selecting a healthy sperm population, means a new revolution in fertility treatments. This selection can be achieved through the technique: magnetic spermatic separation by annexin V (MACS) columns.

The spermatozoa that have initiated the process of apoptosis (aging that leads to programmed cell death), by having the plasma membrane altered, expose the phosphatidylserine (a phospholipid found inside the cytoplasm). Phosphatidylserine has the ability to bind specifically to Annexin V protein. If we incubate the sperm with Annexin V, to which we have previously coupled small metal spheres, it will result in the membrane of spermatozoa that have initiated the process of apoptosis sticking proteins attached to metal spheres.
In the next stage, we pass the seminal sample through a magnetic field, causing the sperm with metal in its membrane to adhere to the magnet. On the other hand, healthy sperm will pass and be free. In this way, we can effectively separate the sperm that have started the process of apoptosis and those that have fragmented DNA, of which they are completely healthy.

The use of a population of non-apoptotic sperm cells with intact membranes and non-fragmented DNA can optimize the results of assisted reproduction techniques and become an effective therapeutic alternative in those cases in which there is a failure of fertilization, low embryonic quality, repeated abortions and sterility without apparent cause.

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